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pt2 shp53 gfp4 addgene  (Addgene inc)


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    Addgene inc pt2 shp53 gfp4 addgene
    Pt2 Shp53 Gfp4 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pt2+shp53+gfp4+addgene/pT2%2Fshp53%2FGFP4+(Plasmid+%2320208)/pm39891906-265-174-175
    Average 93 stars, based on 17 article reviews
    pt2 shp53 gfp4 addgene - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Bradford Assay:

    Article Title: Nuclear-localized HKDC1 promotes hepatocellular carcinoma through phosphorylating RBBP5 to upregulate H3K4me3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Bradford Assay Kit Sangon Biotech Cat# C503041 Hyperactive Universal CUT&Tag Assay Kit Vazyme Cat# TD903-01 HiScript III RT SuperMix Vazyme Cat# R323 EZ-ChIP Kit Millipore Cat# 17-371 ADP-GloTM Max Assay Kit Promega Cat# V6930 Matrigel BD Cat# 356234 Deposited data WB source data This paper; Mendeley data https://data.mendeley.com/datasets/ r2n58xg54s/1 The mass spectrometry proteomics data This paper PRIDE: PXD059464 CUT&Tag data This paper GEO: GSE275531 RNA-seq data This paper GEO: GSE275644 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-3216 Human: Hep3B ATCC Cat# HB-8064 Human: PLC ATCC Cat# CRL-8024 Experimental models: Organisms/strains Nude Shanghai SLAC Laboratory Animal Co., China N/A C57BL/6 Shanghai SLAC Laboratory Animal Co., China N/A Oligonucleotides For shRNA targeting sequences, see Table S1 This manuscript N/A For qRT-PCR primers, see Table S2 This manuscript N/A For ChIP-qRT-PCR primers, see Table S3 This manuscript N/A Recombinant DNA Plasmid: pSin-3xflag-EF1a This manuscript N/A Plasmid: pLKO.1 puro Sigma Cat# SHC001 Plasmid: pET22b-6xHis This manuscript N/A Plasmid: pGEX-4T1-GST This manuscript N/A Plasmid: pT/Caggs-NRASV12 Addgene Cat# 20205 Plasmid: pT2/shp53/GFP4 Addgene Cat# 20208 Plasmid: PT2/C-Luc//PGK-SB13 Addgene Cat# 20207 Genemutants see plasmids part inMethods details This manuscript N/A Software and algorithms FlowJo Software https://www.flowjo.com ZEISS Software https://www.zeiss.com Integrative Genomics Viewer Software https://www.igv.org TissueFAXS Software https://tissuegnostics.com/ HistoQuest Software https://tissuegnostics.com/ GraphPad Prism 8 Software https://www.graphpad.com/ ImageJ Software https://imagej.nih.gov/ij/

    Western Blot:

    Article Title: Nuclear-localized HKDC1 promotes hepatocellular carcinoma through phosphorylating RBBP5 to upregulate H3K4me3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Bradford Assay Kit Sangon Biotech Cat# C503041 Hyperactive Universal CUT&Tag Assay Kit Vazyme Cat# TD903-01 HiScript III RT SuperMix Vazyme Cat# R323 EZ-ChIP Kit Millipore Cat# 17-371 ADP-GloTM Max Assay Kit Promega Cat# V6930 Matrigel BD Cat# 356234 Deposited data WB source data This paper; Mendeley data https://data.mendeley.com/datasets/ r2n58xg54s/1 The mass spectrometry proteomics data This paper PRIDE: PXD059464 CUT&Tag data This paper GEO: GSE275531 RNA-seq data This paper GEO: GSE275644 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-3216 Human: Hep3B ATCC Cat# HB-8064 Human: PLC ATCC Cat# CRL-8024 Experimental models: Organisms/strains Nude Shanghai SLAC Laboratory Animal Co., China N/A C57BL/6 Shanghai SLAC Laboratory Animal Co., China N/A Oligonucleotides For shRNA targeting sequences, see Table S1 This manuscript N/A For qRT-PCR primers, see Table S2 This manuscript N/A For ChIP-qRT-PCR primers, see Table S3 This manuscript N/A Recombinant DNA Plasmid: pSin-3xflag-EF1a This manuscript N/A Plasmid: pLKO.1 puro Sigma Cat# SHC001 Plasmid: pET22b-6xHis This manuscript N/A Plasmid: pGEX-4T1-GST This manuscript N/A Plasmid: pT/Caggs-NRASV12 Addgene Cat# 20205 Plasmid: pT2/shp53/GFP4 Addgene Cat# 20208 Plasmid: PT2/C-Luc//PGK-SB13 Addgene Cat# 20207 Genemutants see plasmids part inMethods details This manuscript N/A Software and algorithms FlowJo Software https://www.flowjo.com ZEISS Software https://www.zeiss.com Integrative Genomics Viewer Software https://www.igv.org TissueFAXS Software https://tissuegnostics.com/ HistoQuest Software https://tissuegnostics.com/ GraphPad Prism 8 Software https://www.graphpad.com/ ImageJ Software https://imagej.nih.gov/ij/

    Mass Spectrometry:

    Article Title: Nuclear-localized HKDC1 promotes hepatocellular carcinoma through phosphorylating RBBP5 to upregulate H3K4me3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Bradford Assay Kit Sangon Biotech Cat# C503041 Hyperactive Universal CUT&Tag Assay Kit Vazyme Cat# TD903-01 HiScript III RT SuperMix Vazyme Cat# R323 EZ-ChIP Kit Millipore Cat# 17-371 ADP-GloTM Max Assay Kit Promega Cat# V6930 Matrigel BD Cat# 356234 Deposited data WB source data This paper; Mendeley data https://data.mendeley.com/datasets/ r2n58xg54s/1 The mass spectrometry proteomics data This paper PRIDE: PXD059464 CUT&Tag data This paper GEO: GSE275531 RNA-seq data This paper GEO: GSE275644 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-3216 Human: Hep3B ATCC Cat# HB-8064 Human: PLC ATCC Cat# CRL-8024 Experimental models: Organisms/strains Nude Shanghai SLAC Laboratory Animal Co., China N/A C57BL/6 Shanghai SLAC Laboratory Animal Co., China N/A Oligonucleotides For shRNA targeting sequences, see Table S1 This manuscript N/A For qRT-PCR primers, see Table S2 This manuscript N/A For ChIP-qRT-PCR primers, see Table S3 This manuscript N/A Recombinant DNA Plasmid: pSin-3xflag-EF1a This manuscript N/A Plasmid: pLKO.1 puro Sigma Cat# SHC001 Plasmid: pET22b-6xHis This manuscript N/A Plasmid: pGEX-4T1-GST This manuscript N/A Plasmid: pT/Caggs-NRASV12 Addgene Cat# 20205 Plasmid: pT2/shp53/GFP4 Addgene Cat# 20208 Plasmid: PT2/C-Luc//PGK-SB13 Addgene Cat# 20207 Genemutants see plasmids part inMethods details This manuscript N/A Software and algorithms FlowJo Software https://www.flowjo.com ZEISS Software https://www.zeiss.com Integrative Genomics Viewer Software https://www.igv.org TissueFAXS Software https://tissuegnostics.com/ HistoQuest Software https://tissuegnostics.com/ GraphPad Prism 8 Software https://www.graphpad.com/ ImageJ Software https://imagej.nih.gov/ij/

    RNA Sequencing:

    Article Title: Nuclear-localized HKDC1 promotes hepatocellular carcinoma through phosphorylating RBBP5 to upregulate H3K4me3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Bradford Assay Kit Sangon Biotech Cat# C503041 Hyperactive Universal CUT&Tag Assay Kit Vazyme Cat# TD903-01 HiScript III RT SuperMix Vazyme Cat# R323 EZ-ChIP Kit Millipore Cat# 17-371 ADP-GloTM Max Assay Kit Promega Cat# V6930 Matrigel BD Cat# 356234 Deposited data WB source data This paper; Mendeley data https://data.mendeley.com/datasets/ r2n58xg54s/1 The mass spectrometry proteomics data This paper PRIDE: PXD059464 CUT&Tag data This paper GEO: GSE275531 RNA-seq data This paper GEO: GSE275644 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-3216 Human: Hep3B ATCC Cat# HB-8064 Human: PLC ATCC Cat# CRL-8024 Experimental models: Organisms/strains Nude Shanghai SLAC Laboratory Animal Co., China N/A C57BL/6 Shanghai SLAC Laboratory Animal Co., China N/A Oligonucleotides For shRNA targeting sequences, see Table S1 This manuscript N/A For qRT-PCR primers, see Table S2 This manuscript N/A For ChIP-qRT-PCR primers, see Table S3 This manuscript N/A Recombinant DNA Plasmid: pSin-3xflag-EF1a This manuscript N/A Plasmid: pLKO.1 puro Sigma Cat# SHC001 Plasmid: pET22b-6xHis This manuscript N/A Plasmid: pGEX-4T1-GST This manuscript N/A Plasmid: pT/Caggs-NRASV12 Addgene Cat# 20205 Plasmid: pT2/shp53/GFP4 Addgene Cat# 20208 Plasmid: PT2/C-Luc//PGK-SB13 Addgene Cat# 20207 Genemutants see plasmids part inMethods details This manuscript N/A Software and algorithms FlowJo Software https://www.flowjo.com ZEISS Software https://www.zeiss.com Integrative Genomics Viewer Software https://www.igv.org TissueFAXS Software https://tissuegnostics.com/ HistoQuest Software https://tissuegnostics.com/ GraphPad Prism 8 Software https://www.graphpad.com/ ImageJ Software https://imagej.nih.gov/ij/

    Planar Chromatography:

    Article Title: Nuclear-localized HKDC1 promotes hepatocellular carcinoma through phosphorylating RBBP5 to upregulate H3K4me3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Bradford Assay Kit Sangon Biotech Cat# C503041 Hyperactive Universal CUT&Tag Assay Kit Vazyme Cat# TD903-01 HiScript III RT SuperMix Vazyme Cat# R323 EZ-ChIP Kit Millipore Cat# 17-371 ADP-GloTM Max Assay Kit Promega Cat# V6930 Matrigel BD Cat# 356234 Deposited data WB source data This paper; Mendeley data https://data.mendeley.com/datasets/ r2n58xg54s/1 The mass spectrometry proteomics data This paper PRIDE: PXD059464 CUT&Tag data This paper GEO: GSE275531 RNA-seq data This paper GEO: GSE275644 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-3216 Human: Hep3B ATCC Cat# HB-8064 Human: PLC ATCC Cat# CRL-8024 Experimental models: Organisms/strains Nude Shanghai SLAC Laboratory Animal Co., China N/A C57BL/6 Shanghai SLAC Laboratory Animal Co., China N/A Oligonucleotides For shRNA targeting sequences, see Table S1 This manuscript N/A For qRT-PCR primers, see Table S2 This manuscript N/A For ChIP-qRT-PCR primers, see Table S3 This manuscript N/A Recombinant DNA Plasmid: pSin-3xflag-EF1a This manuscript N/A Plasmid: pLKO.1 puro Sigma Cat# SHC001 Plasmid: pET22b-6xHis This manuscript N/A Plasmid: pGEX-4T1-GST This manuscript N/A Plasmid: pT/Caggs-NRASV12 Addgene Cat# 20205 Plasmid: pT2/shp53/GFP4 Addgene Cat# 20208 Plasmid: PT2/C-Luc//PGK-SB13 Addgene Cat# 20207 Genemutants see plasmids part inMethods details This manuscript N/A Software and algorithms FlowJo Software https://www.flowjo.com ZEISS Software https://www.zeiss.com Integrative Genomics Viewer Software https://www.igv.org TissueFAXS Software https://tissuegnostics.com/ HistoQuest Software https://tissuegnostics.com/ GraphPad Prism 8 Software https://www.graphpad.com/ ImageJ Software https://imagej.nih.gov/ij/

    shRNA:

    Article Title: Nuclear-localized HKDC1 promotes hepatocellular carcinoma through phosphorylating RBBP5 to upregulate H3K4me3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Bradford Assay Kit Sangon Biotech Cat# C503041 Hyperactive Universal CUT&Tag Assay Kit Vazyme Cat# TD903-01 HiScript III RT SuperMix Vazyme Cat# R323 EZ-ChIP Kit Millipore Cat# 17-371 ADP-GloTM Max Assay Kit Promega Cat# V6930 Matrigel BD Cat# 356234 Deposited data WB source data This paper; Mendeley data https://data.mendeley.com/datasets/ r2n58xg54s/1 The mass spectrometry proteomics data This paper PRIDE: PXD059464 CUT&Tag data This paper GEO: GSE275531 RNA-seq data This paper GEO: GSE275644 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-3216 Human: Hep3B ATCC Cat# HB-8064 Human: PLC ATCC Cat# CRL-8024 Experimental models: Organisms/strains Nude Shanghai SLAC Laboratory Animal Co., China N/A C57BL/6 Shanghai SLAC Laboratory Animal Co., China N/A Oligonucleotides For shRNA targeting sequences, see Table S1 This manuscript N/A For qRT-PCR primers, see Table S2 This manuscript N/A For ChIP-qRT-PCR primers, see Table S3 This manuscript N/A Recombinant DNA Plasmid: pSin-3xflag-EF1a This manuscript N/A Plasmid: pLKO.1 puro Sigma Cat# SHC001 Plasmid: pET22b-6xHis This manuscript N/A Plasmid: pGEX-4T1-GST This manuscript N/A Plasmid: pT/Caggs-NRASV12 Addgene Cat# 20205 Plasmid: pT2/shp53/GFP4 Addgene Cat# 20208 Plasmid: PT2/C-Luc//PGK-SB13 Addgene Cat# 20207 Genemutants see plasmids part inMethods details This manuscript N/A Software and algorithms FlowJo Software https://www.flowjo.com ZEISS Software https://www.zeiss.com Integrative Genomics Viewer Software https://www.igv.org TissueFAXS Software https://tissuegnostics.com/ HistoQuest Software https://tissuegnostics.com/ GraphPad Prism 8 Software https://www.graphpad.com/ ImageJ Software https://imagej.nih.gov/ij/

    Quantitative RT-PCR:

    Article Title: Nuclear-localized HKDC1 promotes hepatocellular carcinoma through phosphorylating RBBP5 to upregulate H3K4me3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Bradford Assay Kit Sangon Biotech Cat# C503041 Hyperactive Universal CUT&Tag Assay Kit Vazyme Cat# TD903-01 HiScript III RT SuperMix Vazyme Cat# R323 EZ-ChIP Kit Millipore Cat# 17-371 ADP-GloTM Max Assay Kit Promega Cat# V6930 Matrigel BD Cat# 356234 Deposited data WB source data This paper; Mendeley data https://data.mendeley.com/datasets/ r2n58xg54s/1 The mass spectrometry proteomics data This paper PRIDE: PXD059464 CUT&Tag data This paper GEO: GSE275531 RNA-seq data This paper GEO: GSE275644 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-3216 Human: Hep3B ATCC Cat# HB-8064 Human: PLC ATCC Cat# CRL-8024 Experimental models: Organisms/strains Nude Shanghai SLAC Laboratory Animal Co., China N/A C57BL/6 Shanghai SLAC Laboratory Animal Co., China N/A Oligonucleotides For shRNA targeting sequences, see Table S1 This manuscript N/A For qRT-PCR primers, see Table S2 This manuscript N/A For ChIP-qRT-PCR primers, see Table S3 This manuscript N/A Recombinant DNA Plasmid: pSin-3xflag-EF1a This manuscript N/A Plasmid: pLKO.1 puro Sigma Cat# SHC001 Plasmid: pET22b-6xHis This manuscript N/A Plasmid: pGEX-4T1-GST This manuscript N/A Plasmid: pT/Caggs-NRASV12 Addgene Cat# 20205 Plasmid: pT2/shp53/GFP4 Addgene Cat# 20208 Plasmid: PT2/C-Luc//PGK-SB13 Addgene Cat# 20207 Genemutants see plasmids part inMethods details This manuscript N/A Software and algorithms FlowJo Software https://www.flowjo.com ZEISS Software https://www.zeiss.com Integrative Genomics Viewer Software https://www.igv.org TissueFAXS Software https://tissuegnostics.com/ HistoQuest Software https://tissuegnostics.com/ GraphPad Prism 8 Software https://www.graphpad.com/ ImageJ Software https://imagej.nih.gov/ij/

    Chromatin Immunoprecipitation:

    Article Title: Nuclear-localized HKDC1 promotes hepatocellular carcinoma through phosphorylating RBBP5 to upregulate H3K4me3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Bradford Assay Kit Sangon Biotech Cat# C503041 Hyperactive Universal CUT&Tag Assay Kit Vazyme Cat# TD903-01 HiScript III RT SuperMix Vazyme Cat# R323 EZ-ChIP Kit Millipore Cat# 17-371 ADP-GloTM Max Assay Kit Promega Cat# V6930 Matrigel BD Cat# 356234 Deposited data WB source data This paper; Mendeley data https://data.mendeley.com/datasets/ r2n58xg54s/1 The mass spectrometry proteomics data This paper PRIDE: PXD059464 CUT&Tag data This paper GEO: GSE275531 RNA-seq data This paper GEO: GSE275644 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-3216 Human: Hep3B ATCC Cat# HB-8064 Human: PLC ATCC Cat# CRL-8024 Experimental models: Organisms/strains Nude Shanghai SLAC Laboratory Animal Co., China N/A C57BL/6 Shanghai SLAC Laboratory Animal Co., China N/A Oligonucleotides For shRNA targeting sequences, see Table S1 This manuscript N/A For qRT-PCR primers, see Table S2 This manuscript N/A For ChIP-qRT-PCR primers, see Table S3 This manuscript N/A Recombinant DNA Plasmid: pSin-3xflag-EF1a This manuscript N/A Plasmid: pLKO.1 puro Sigma Cat# SHC001 Plasmid: pET22b-6xHis This manuscript N/A Plasmid: pGEX-4T1-GST This manuscript N/A Plasmid: pT/Caggs-NRASV12 Addgene Cat# 20205 Plasmid: pT2/shp53/GFP4 Addgene Cat# 20208 Plasmid: PT2/C-Luc//PGK-SB13 Addgene Cat# 20207 Genemutants see plasmids part inMethods details This manuscript N/A Software and algorithms FlowJo Software https://www.flowjo.com ZEISS Software https://www.zeiss.com Integrative Genomics Viewer Software https://www.igv.org TissueFAXS Software https://tissuegnostics.com/ HistoQuest Software https://tissuegnostics.com/ GraphPad Prism 8 Software https://www.graphpad.com/ ImageJ Software https://imagej.nih.gov/ij/

    Recombinant:

    Article Title: Nuclear-localized HKDC1 promotes hepatocellular carcinoma through phosphorylating RBBP5 to upregulate H3K4me3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Bradford Assay Kit Sangon Biotech Cat# C503041 Hyperactive Universal CUT&Tag Assay Kit Vazyme Cat# TD903-01 HiScript III RT SuperMix Vazyme Cat# R323 EZ-ChIP Kit Millipore Cat# 17-371 ADP-GloTM Max Assay Kit Promega Cat# V6930 Matrigel BD Cat# 356234 Deposited data WB source data This paper; Mendeley data https://data.mendeley.com/datasets/ r2n58xg54s/1 The mass spectrometry proteomics data This paper PRIDE: PXD059464 CUT&Tag data This paper GEO: GSE275531 RNA-seq data This paper GEO: GSE275644 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-3216 Human: Hep3B ATCC Cat# HB-8064 Human: PLC ATCC Cat# CRL-8024 Experimental models: Organisms/strains Nude Shanghai SLAC Laboratory Animal Co., China N/A C57BL/6 Shanghai SLAC Laboratory Animal Co., China N/A Oligonucleotides For shRNA targeting sequences, see Table S1 This manuscript N/A For qRT-PCR primers, see Table S2 This manuscript N/A For ChIP-qRT-PCR primers, see Table S3 This manuscript N/A Recombinant DNA Plasmid: pSin-3xflag-EF1a This manuscript N/A Plasmid: pLKO.1 puro Sigma Cat# SHC001 Plasmid: pET22b-6xHis This manuscript N/A Plasmid: pGEX-4T1-GST This manuscript N/A Plasmid: pT/Caggs-NRASV12 Addgene Cat# 20205 Plasmid: pT2/shp53/GFP4 Addgene Cat# 20208 Plasmid: PT2/C-Luc//PGK-SB13 Addgene Cat# 20207 Genemutants see plasmids part inMethods details This manuscript N/A Software and algorithms FlowJo Software https://www.flowjo.com ZEISS Software https://www.zeiss.com Integrative Genomics Viewer Software https://www.igv.org TissueFAXS Software https://tissuegnostics.com/ HistoQuest Software https://tissuegnostics.com/ GraphPad Prism 8 Software https://www.graphpad.com/ ImageJ Software https://imagej.nih.gov/ij/

    Plasmid Preparation:

    Article Title: Nuclear-localized HKDC1 promotes hepatocellular carcinoma through phosphorylating RBBP5 to upregulate H3K4me3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Bradford Assay Kit Sangon Biotech Cat# C503041 Hyperactive Universal CUT&Tag Assay Kit Vazyme Cat# TD903-01 HiScript III RT SuperMix Vazyme Cat# R323 EZ-ChIP Kit Millipore Cat# 17-371 ADP-GloTM Max Assay Kit Promega Cat# V6930 Matrigel BD Cat# 356234 Deposited data WB source data This paper; Mendeley data https://data.mendeley.com/datasets/ r2n58xg54s/1 The mass spectrometry proteomics data This paper PRIDE: PXD059464 CUT&Tag data This paper GEO: GSE275531 RNA-seq data This paper GEO: GSE275644 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-3216 Human: Hep3B ATCC Cat# HB-8064 Human: PLC ATCC Cat# CRL-8024 Experimental models: Organisms/strains Nude Shanghai SLAC Laboratory Animal Co., China N/A C57BL/6 Shanghai SLAC Laboratory Animal Co., China N/A Oligonucleotides For shRNA targeting sequences, see Table S1 This manuscript N/A For qRT-PCR primers, see Table S2 This manuscript N/A For ChIP-qRT-PCR primers, see Table S3 This manuscript N/A Recombinant DNA Plasmid: pSin-3xflag-EF1a This manuscript N/A Plasmid: pLKO.1 puro Sigma Cat# SHC001 Plasmid: pET22b-6xHis This manuscript N/A Plasmid: pGEX-4T1-GST This manuscript N/A Plasmid: pT/Caggs-NRASV12 Addgene Cat# 20205 Plasmid: pT2/shp53/GFP4 Addgene Cat# 20208 Plasmid: PT2/C-Luc//PGK-SB13 Addgene Cat# 20207 Genemutants see plasmids part inMethods details This manuscript N/A Software and algorithms FlowJo Software https://www.flowjo.com ZEISS Software https://www.zeiss.com Integrative Genomics Viewer Software https://www.igv.org TissueFAXS Software https://tissuegnostics.com/ HistoQuest Software https://tissuegnostics.com/ GraphPad Prism 8 Software https://www.graphpad.com/ ImageJ Software https://imagej.nih.gov/ij/

    Software:

    Article Title: Nuclear-localized HKDC1 promotes hepatocellular carcinoma through phosphorylating RBBP5 to upregulate H3K4me3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Bradford Assay Kit Sangon Biotech Cat# C503041 Hyperactive Universal CUT&Tag Assay Kit Vazyme Cat# TD903-01 HiScript III RT SuperMix Vazyme Cat# R323 EZ-ChIP Kit Millipore Cat# 17-371 ADP-GloTM Max Assay Kit Promega Cat# V6930 Matrigel BD Cat# 356234 Deposited data WB source data This paper; Mendeley data https://data.mendeley.com/datasets/ r2n58xg54s/1 The mass spectrometry proteomics data This paper PRIDE: PXD059464 CUT&Tag data This paper GEO: GSE275531 RNA-seq data This paper GEO: GSE275644 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-3216 Human: Hep3B ATCC Cat# HB-8064 Human: PLC ATCC Cat# CRL-8024 Experimental models: Organisms/strains Nude Shanghai SLAC Laboratory Animal Co., China N/A C57BL/6 Shanghai SLAC Laboratory Animal Co., China N/A Oligonucleotides For shRNA targeting sequences, see Table S1 This manuscript N/A For qRT-PCR primers, see Table S2 This manuscript N/A For ChIP-qRT-PCR primers, see Table S3 This manuscript N/A Recombinant DNA Plasmid: pSin-3xflag-EF1a This manuscript N/A Plasmid: pLKO.1 puro Sigma Cat# SHC001 Plasmid: pET22b-6xHis This manuscript N/A Plasmid: pGEX-4T1-GST This manuscript N/A Plasmid: pT/Caggs-NRASV12 Addgene Cat# 20205 Plasmid: pT2/shp53/GFP4 Addgene Cat# 20208 Plasmid: PT2/C-Luc//PGK-SB13 Addgene Cat# 20207 Genemutants see plasmids part inMethods details This manuscript N/A Software and algorithms FlowJo Software https://www.flowjo.com ZEISS Software https://www.zeiss.com Integrative Genomics Viewer Software https://www.igv.org TissueFAXS Software https://tissuegnostics.com/ HistoQuest Software https://tissuegnostics.com/ GraphPad Prism 8 Software https://www.graphpad.com/ ImageJ Software https://imagej.nih.gov/ij/



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